Electrophoresis: DNA separation
Electrophoresis is a method of separation of DNA fragments that aims at the mobility of these fragments along a gel that receives the application of an electric current.
How it happens
The negatively charged DNA drives through the gel to the positively charged pole. The fragments are separated by size, with those that are lighter and smaller run in front and the largest and heaviest behind.
Types of Electrophoresis Gels
Gels can be of two types: Argarose (natural polysaccharide) or Polyacrylamide (synthetic matrix).
The difference between these two types of gels is in the coloration when viewing the product of PCR.
Did you know?
“The main way to identify nucleic acids is by the electrophoresis method.”